Human intestinal gas measurement systems: in vitro fermentation and gas capsules. 人体肠道气体测量系统：体外发酵和气体胶囊
Abstract The biological and clinical significance of the human gut microbiome is currently attracting worldwide attention. While rRNA and DNA technologies led to a quantum leap in our understanding of the numbers and types of gut microorganisms, much less is known about these microorganisms' activity in situ and in real time. Accurately measuring their byproducts, including intestinal gases, may offer unique biomarkers for specific gut microbiota, accelerating our understanding of the relationships among intestinal gases, the metabolic activity of the gut microbiome, and human health states. Here we present two novel techniques, namely in vitro fermentation and gas capsule systems, for measuring and assessing selected gas species. We discuss new developments with these technologies and the methods of their implementation and provide an overall review of their operation. 人肠道微生物组的生物学和临床意义目前正引起全世界的关注。虽然rRNA和DNA技术使得我们在对肠道微生物的数量和种类的研究方面取得了巨大的飞跃，但对这些微生物原位和实时活动知之甚少。准确测量其副产物，包括肠道气体，可以为特定的肠道微生物群提供独特的生物标记，加速我们对肠道气体、肠道微生物组的代谢活动和人类健康状态之间关系的理解。 在这里，我们提出了两种新技术，即体外发酵和气体胶囊系统，用于测量和评估选定的气体种类。 我们将讨论这些技术的新发展及其实施方法，并对其运作进行全面审查。
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